Fig. 3. LC3 puncta in podocytes treated with Ang II. Bafilomycin A1 (10 nM) and chloroquine (CQ, 0.1 mM) increased LC3 puncta and additional Ang II further increased the expression of LC3 puncta significantly (A). Magnification, ×200; Bar = 10 μm. LC3 puncta fluorescence (mean ± SD) were quantified for each experiment (n = 4; B). At least 30 cells were counted in each individual experiment. Ang II increases LC3 puncta in time- and concentration-dependent manners (C and D). At 12 hours of incubation with Ang II, a concentration-dependent increase in LC3 expression is observed (E). 10⁻⁶ M Ang II also increases LC3 puncta in a time-dependent manner; however, LC3 puncta levels decreased at 24 hours (F). Nuclei are colored in blue by DAPI. Data on the analysis LC3 puncta ratio of podocytes are presented as mean ± SD (n = 3 without notice). Control (100%); compared to conditions without Ang II. ^*P<0.05 and ^**P<0.01 versus control. ^＃＃P<0.01 versus condition with Ang II.